COURSE DESCRIPTION AND APPLICATION INFORMATION
| Course Name | Code | Semester | T+A+L (hour/week) | Type (C / O) | Local Credit | ECTS |
|---|---|---|---|---|---|---|
| Cell and Tissue Culture | MBG 419 | Fall | 03+00+00 | Elective | 3 | 5 |
| Academic Unit: | Molecular Biology and Genetics |
| Mode of Delivery: | Face to face |
| Prerequisites: | - |
| Language of Instruction: | English |
| Level of Course Unit: | Undergraduate |
| Course Coordinator: | - - |
| Course Objectives: | To teach the standard methods used in cell and tissue culture as a powerful tool to investigate the biology of cells, especially in drug discovery. |
| Course Contents: | Animal Cell Culture Biology and Microscopy of Cultured Cells Basic Cell Culture Equipment, Reagents, and Techniques Aseptic Technique, Sterilization, and introduction to GCCP Maintenance of Cell Lines Cell Line Characterization Fundamentals of Cancer Cell Culture Functional Assays in Cell Culture Cell Culture Contamination 3D Cell Culture Culture of Specific Cell Types |
| Learning Outcomes of the Course Unit (LO): |
|
| Planned Learning Activities and Teaching Methods: | PowerPoint presentations, including important images and daily examples about the subject, which are also shared with the students through the KHAS Learn platform. Explaining the concepts on the board (students can take photos). Watching mechanistic/explanatory videos during lectures and discussing with students. Discussion with the students during the lecture. Questions integrating the previously covered lectures into ongoing lecture content will allow students better understand how the concepts are related to each other. JoVE platform, selected review/ research articles will support the course content and help students review the lecture and visualize the subject. |
WEEKLY SUBJECTS AND RELATED PREPARATIONS
| Week | Subjects | Related Preperation |
|---|---|---|
| 1 | Animal Cell Culture: Past, Present, and Future | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 2 | Biology and Microscopy of Cultured Cells | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 3 | Basic Cell Culture Equipment and Matter | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 4 | Basic Cell Culture Reagents | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 5 | Aseptic Technique, Sterilization, and introduction to GCCP | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 6 | Basic Cell Culture Techniques | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 7 | Maintenance of Cell Lines | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 8 | Cell Line Characterization | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 9 | Fundamentals of Cancer Cell Culture | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 10 | Functional Assays in Cell Culture | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 11 | Cell Culture Contamination | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 12 | 3D Cell Culture | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 13 | Culture of Specific Cell Types | The students should follow the KHAS Learn pages to examine the assigned material before and after the lecture hours. |
| 14 | Student Presentations | Students should be prepared according to the shared rules and format. |
At Kadir Has University, a Semester is 14 weeks; The weeks 15 and 16 are reserved for final exams.
REQUIRED AND RECOMMENDED READING
| Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (Seventh Edition) by R. Ian Freshney (ISBN-13: 978-1118873656) Basic Cell Culture (Practical Approach Series) (Second Edition) edited by J. M. Davis (ISBN-13: 978-0199638536) Cancer Cell Culture (Methods and Protocols) edited by Simon P. Langdon (E-ISBN: 1-59259-406-9) |
OTHER COURSE RESOURCES
| JoVE Scientific Video Journal will be used to support the lecture visually. The links will be available on the KHAS Learn page of the course. Please activate your JOVE accounts in order to have full access to the links. Your KHAS e-mail and password will be your username and password, respectively. |
ASSESSMENT METHODS AND CRITERIA
| Semester Requirements | Number | Percentage of Grade (%) |
|---|---|---|
| Attendance / Participation | 14 | - |
| Homework Assignments | 1 | 20 |
| Presentation / Jury | 1 | 20 |
| Extra-Class Activities (reading, individual study etc.) | 10 | 20 |
| Final Exam | 1 | 40 |
| Total: | 27 | 100 |
WORKLOAD
| Events | Count | Duration (Hours) | Total Workload (hour) |
|---|---|---|---|
| Course Hours | 14 | 3 | 42 |
| Homework Assigments | 1 | 20 | 20 |
| Preparation for Presentation / Jury | 1 | 20 | 20 |
| Extra-Class Activities (reading,individiual work, etc.) | 10 | 2 | 20 |
| Final Exam | 1 | 23 | 23 |
| Total Workload (hour): | 125 | ||
1 ECTS = 25 Hours Workload
THE RELATIONSHIP BETWEEN COURSE LEARNING OUTCOMES (LO) AND PROGRAM QUALIFICATIONS (PQ)
| # | PQ1 | PQ2 | PQ3 | PQ4 | PQ5 | PQ6 | PQ7 | PQ8 | PQ9 | PQ10 |
| LO1 | ||||||||||
| LO2 | ||||||||||
| LO3 | ||||||||||
| LO4 |
Contribution: 1 Low, 2 Average, 3 High